Superantigen Protocols
Teresa Krakauer
ΡΠ΅Π². 2008β―Π³. Β· Methods in molecular biology ΠΠ½ΠΈΠ³Π° 214 Β· Springer Science & Business Media
Π-ΠΊΠ½ΠΈΠ³Π°
260
Π‘ΡΡΠ°Π½ΠΈΡΠΈ
reportΠΡΠ΅Π½ΠΈΡΠ΅ ΠΈ ΡΠ΅ΡΠ΅Π½Π·ΠΈΠΈΡΠ΅ Π½Π΅ ΡΠ΅ ΠΏΠΎΡΠ²ΡΠ΄Π΅Π½ΠΈ Β ΠΠΎΠ·Π½Π°ΡΡΠ΅ ΠΏΠΎΠ²Π΅ΡΠ΅
ΠΠ° Π΅-ΠΊΠ½ΠΈΠ³Π°Π²Π°
Superantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of these potent activators of the immune system. The protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. Additional techniques for the discovery of new activators of the immune system, the development of new therapeutics for the suppression of superantigen-induced effects, and the treatment of inflammatory diseases are detailed.
Comprehensive and cutting-edge, Superantigen Protocols provides a broad range of novel immunological and biochemical techniques that can be successfully applied not only to the structural, functional, and clinical properties of superantigens, but also to the many varieties of such proteins and to new cellular systems.
Comprehensive and cutting-edge, Superantigen Protocols provides a broad range of novel immunological and biochemical techniques that can be successfully applied not only to the structural, functional, and clinical properties of superantigens, but also to the many varieties of such proteins and to new cellular systems.
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ΠΠ° Π΄Π° ΡΠΈΡΠ°ΡΠ΅ Π½Π° ΡΡΠ΅Π΄ΠΈ ΡΠΎ Π΅-ΠΌΠ°ΡΡΠΈΠ»ΠΎ, ΠΊΠ°ΠΊΠΎ ΡΡΠΎ ΡΠ΅ Π΅-ΡΠΈΡΠ°ΡΠΈΡΠ΅ Kobo, ΡΠ΅ ΡΡΠ΅Π±Π° Π΄Π° ΠΏΡΠ΅Π·Π΅ΠΌΠ΅ΡΠ΅ Π΄Π°ΡΠΎΡΠ΅ΠΊΠ° ΠΈ Π΄Π° ΡΠ° ΠΏΡΠ΅ΡΡΠ»ΠΈΡΠ΅ Π½Π° ΡΡΠ΅Π΄ΠΎΡ. Π‘Π»Π΅Π΄Π΅ΡΠ΅ Π³ΠΈ Π΄Π΅ΡΠ°Π»Π½ΠΈΡΠ΅ ΡΠΏΠ°ΡΡΡΠ²Π° Π²ΠΎ Π¦Π΅Π½ΡΠ°ΡΠΎΡ Π·Π° ΠΏΠΎΠΌΠΎΡ Π·Π° ΠΏΡΠ΅ΡΡΠ»Π°ΡΠ΅ Π½Π° Π΄Π°ΡΠΎΡΠ΅ΠΊΠΈΡΠ΅ Π½Π° ΠΏΠΎΠ΄Π΄ΡΠΆΠ°Π½ΠΈ Π΅-ΡΠΈΡΠ°ΡΠΈ.
