Advanced Biophysical Techniques in Biosciences

Nirmal Mazumder · Rajib Biswas · Guan-Yu Zhuo

Sep 2025 · Springer Nature

Ebook

427

Pages

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About this ebook

This book presents an overview of advanced biophysical techniques that can be used to understand the physicochemical properties of biomolecules and biomaterials and expand their potential for biomedical applications. It is split into two parts, the first covering advanced biophysical techniques and the second covering bioscience applications. Adequate knowledge about the behavior of biomacromolecules is essential for standardizing their applications in various industries. These properties are strongly influenced by the composition, chain structure (e.g. linear or branch), linkage patterns, and molecular weight of the biomolecules. This book describes the various internal and external factors that develop the structural and functional properties of biomolecules. Further, it covers the advanced techniques that can be used to discover and enhance these properties, such as scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, X-ray crystallography, fluorescence spectroscopy, surface plasmon resonance, surface-enhanced Raman spectroscopy, force spectroscopy, optical tweezers, and more. It also covers high-performance liquid chromatography for biomolecule detection, including sample preparation, column selection, mobile phase determination, and the choice of an appropriate detector for the investigation.

This book serves as a modern resource on the topic, providing an in-depth analysis of various important physicochemical properties as well as their wide range of applications, including in pharmaceuticals, bioimaging/sensing, cancer therapy, food sciences, textiles, scaffolds, drug delivery, and tissue engineering. Readers are presented with several invasive and non-invasive techniques that can be used for the characterization of biomacromolecules along with many types of physical, chemical, and physicochemical modifications that can be used to enhance their usage.

About the author

Dr. Nirmal Mazumder is an Associate Professor at the Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE), Manipal, India. He obtained his Ph.D. in 2013 from National Yang Ming Chiao Tung University, Taipei, Taiwan. From 2013 to 2016, he worked as a postdoctoral fellow at the University of Virginia, USA, and the Italian Institute of Technology, Genoa, Italy. He has been developing nonlinear optical microscopes, including two photon fluorescence, second harmonic generation, coherent anti-Stokes Raman scattering for biomedical applications. His research interests include development of microscopy-spectroscopy techniques, biomedical devices, biophotonics, smart materials, microfluidics and its biomedical applications. He has published more than 100 research articles in the peer-reviewed international journals, 8 books, 25 book chapters, and several poster/oral/invited presentations in National and International conferences. He is working on several national and bilateral research projects funded by Government of India as Principal Investigator. He is also working as the reviewer/guest editor/associate editors in several prestigious journals. He is the member of several national and international scientific societies and organizations including, the Optical Society (OPTICA) (Senior member), SPIE --the International Society for Optical Engineering (Senior member), Society of Biological Chemists (I) (Life Member), Environmental Mutagen Society of India (Life Member), Asian Polymer Association (Life Member), Indian Laser Association-ILA (Life Member), Research Society for the Study of Diabetes in India - RSSDI (Life Member).

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